Fig. 2.

A–C: intracellular H₂O₂ responses [change in peroxyfluor 6 (PF6)-AM (PF6-AM) intensity] to an increase in mTAL luminal flow from 5 to 20 nl/min in SS (n = 12), SS^Nox4−/− (n = 8), and SS^{p67phox−/−} (n = 8) rats fed 4.0% NaCl diet for 3 days prior to study (■, ●, and ▲). □, ○, and △, Fluorescence changes of nonperfused mTALs. *Significant differences between slopes of perfused and nonperfused mTALs within each strain [2-way repeated-measures (RM) ANOVA, P_interaction < 0.05]. D: summary of responses to increased luminal flow in mTALs of SS (■), SS^Nox4−/− (●), and SS^{p67phox−/−} (▲) rats. †Significant differences between slopes of SS and SS^Nox4−/− rats; #significant differences between slopes of SS^Nox4−/− and SS^{p67phox−/−} rats (2-way RM ANOVA, P_interaction < 0.05).