Table 1.
Gene expression of Nox1, Cybb (Nox2), Nox3, Nox4, and Ncf2 (p67phox) in isolated components of the nephron from rats with SS genomic background fed 0.4% and 4% NaCl for 7 days
| Nephron Segments |
||||
|---|---|---|---|---|
| Gene Name | mTAL | Glo | PT | OMCD |
| 0.4 % NaCl | ||||
| Nox4 | 3.6 ± 0.6 | 3.0 ± 1.7 | 210.4 ± 75.5 | 1.1 ± 0.3 |
| Ncf2 (p67phox) | 0.2 ± 0.0 | 0.8 ± 0.1 | 0.2 ± 0.1 | 0.3 ± 0.1 |
| Cybb (Nox2) | 3.5 ± 0.3 | 9.9 ± 0.9 | 2.6 ± 0.3 | 3.3 ± 0.4 |
| Nox1 | 0.7 ± 0.1 | 0.5 ± 0.0 | 0.6 ± 0.1 | 1.1 ± 0.1 |
| Nox3 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| 4 % NaCl for 7 days | ||||
| Nox4 | 3.0 ± 0.1 | 3.1 ± 1.2 | 155.9 ± 52.6 | 2.0 ± 0.4 |
| Ncf2 (p67phox) | 0.5 ± 0.1 | 0.8 ± 0.1 | 0.4 ± 0.1 | 0.3 ± 0.1 |
| Cybb (Nox2) | 5.6 ± 0.6 | 11.1 ± 0.2 | 6.1 ± 1.0 | 4.4 ± 0.8 |
| Nox1 | 0.6 ± 0.0 | 0.4 ± 0.1 | 0.7 ± 0.1 | 1.2 ± 0.1 |
| Nox3 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
Values (means ± SE) are expressed as FPKM (fragments per kilobase of transcript per million mapped reads). Gene expression was determined by RNA-seq in isolated components of the nephron from rats with salt-sensitive (SS) genomic background. Glo, glomerulus; mTAL, medullary thick ascending limb of Henle; OMCD, outer medullary collecting duct; PT, proximal tubule.