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. 2004 Sep 16;23(20):4051–4060. doi: 10.1038/sj.emboj.7600385

Figure 3.

Figure 3

Transcription of miRNA genes is sensitive to α-amanitin. HeLa cells were treated with α-amanitin at a concentration of either 0 or 50 μg/ml for 9 h. Total RNA was prepared from these cells and 5 μg of total RNA was subjected to cDNA synthesis in 20 μl reaction. The levels of pri-miRNAs were determined by RT–PCR using gene-specific primers. Different amounts (0.5, 1, or 2 μl) of cDNA template from the untreated cells (lanes 1–3) were used for PCR to see if the PCR amplification is in a quantitative range. GAPDH pre-mRNA, 45S pre-rRNA, and pre-tRNAtyr were used as controls.