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. 2004 Sep 16;23(20):4051–4060. doi: 10.1038/sj.emboj.7600385

Figure 6.

Figure 6

Transcription of mir-23a∼27a∼24-2 gene cluster is dependent on pol II. (A) Luciferase assay was performed using the reporter plasmid pGL3-23P639 or pRL-CMV. HEK293T cells were transfected with the reporter plasmids. Right after adding the plasmids, the cells were incubated in 0 or 50 μg/ml of α-amanitin for 6 h before the cells were harvested for assay. (B) RT–PCR to show that pol II activity (GAPDH pre-mRNA) is selectively reduced under this condition while pol III activity (pre-tRNAtyr) is not affected.