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. 2004 Sep 23;23(20):3950–3961. doi: 10.1038/sj.emboj.7600387

Figure 1.

Figure 1

Cellular localisation of FRB-βarrestin2-YFP when coexpressed with either Myc-V2R-FKBP or HA-V1aR-FKBP following AVP or AP21967 treatment. Schematic representation of AP21967-induced translocation of FRB-βarrestin2-YFP to Myc-V2R-FKBP or HA-V1aR-FKBP (A). HEK 293T cells cotransfected with Myc-V2R-FKBP+FRB-βarrestin2-YFP (B) or HA-V1aR-FKBP+FRB-βarrestin2-YFP (C) were incubated with rabbit polyclonal antibody A14 or mouse monoclonal antibody 12CA5, for 1 h at 4°C. Next, cells were treated or not for 30 min at 37°C with the indicated ligand (100 nM AVP or 500 nM AP21967), fixed, permeabilised and labelled with goat anti-rabbit (V2R) or anti-mouse (V1aR) antibody coupled to Texas red. The samples were analysed by confocal laser-scanning microscopy.