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. 2004 Sep 23;23(20):3950–3961. doi: 10.1038/sj.emboj.7600387

Figure 3.

Figure 3

Kinetics of Myc-V2R-FKBP and HA-V1aR-FKBP endocytosis. HEK 293T cells coexpressing Myc-V2R-FKBP+FRB-βarrestin2-YFP (A) or HA-V1aR-FKBP+FRB-βarrestin2-YFP (B) were treated or not with 100 nM AVP or 500 nM AP21967 at 37°C for the indicated periods of time. In (C), the cells were treated for 30 min at 37°C with 100 nM AVP and 25 μM AP21967 added separately or in combination. The cell surface Myc epitope-tagged V2R and HA epitope-tagged V1aR were detected by ELISA. The extent of internalisation was determined by measuring the cell surface receptor before and after ligand treatment and expressed as the loss of cell surface expression (% of control). All values correspond to the mean±s.e.m. calculated from three independent experiments.