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. 2004 Sep 23;23(20):3950–3961. doi: 10.1038/sj.emboj.7600387

Figure 8.

Figure 8

Conditional plasma membrane translocation of FRB-βarrestin2-YFP to myristoylated FKBP2 and ERK1/2 activation. HEK 293T cells cotransfected with MyrFKBP2+FRB-βarrestin2-YFP were treated with 500 nM AP21967 at 37°C for the indicated periods of time, so as to induce the plasma membrane translocation of FRB-βarrestin2-YFP. Both the cellular localisation of FRB-βarrestin2-YFP (A) and the ERK1/2 activation (B) were then determined by confocal microscopy and immunoblotting with a phospho-ERK1/2-specific antibody (P-ERK1/2), respectively. Expression levels of ERK1/2 were controlled using an antibody directed against the total kinase population (ERK1/2). The levels of phosphorylated ERK1/2 are expressed as the percentage of the maximal increase of phosphorylation obtained during the time course. The graphs represent the mean±s.e.m. calculated from six independent experiments.