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. 2004 Sep 23;23(20):4061–4071. doi: 10.1038/sj.emboj.7600402

Figure 5.

Figure 5

SUZ12 is required for proliferation of both normal and tumor cells. (A) Colony formation of U2OS cells stably interfered with empty (CTRL) and SUZ12 shRNA (SUZ12) retroviral vectors showing the proliferative impairment of SUZ12 interfered U2OS cells. In the bottom panels, WB analyses of SUZ12 levels of the same cells are presented. β-Tubulin was used as loading control. (B–D) Colony, growth curves and BrdU FACS analysis of primary human TIG3-T cells stably interfered with empty (CTRL) and SUZ12 shRNA (SUZ12) retroviral vectors, showing the requirement of SUZ12 for proliferation of diploid human cells. (E) WB analysis of TIG3-T cells at each day of the experiment presented in (C), showing the efficiency of the SUZ12 interference and the reduction of the EZH2 protein levels. β-Tubulin served as loading control. (F) Serum induction of serum-starved human diploid fibroblasts. WB analyses show the accumulation of both SUZ12 and cyclin A2 at the G1–S transition. β-Tubulin was used as loading control. (G) SUZ12 is required for S-phase entry. BrdU incorporation was measured with (24 h) and without (0 h) serum induction of serum-starved TIG3-T cells stably interfered with empty (CTRL) and SUZ12 shRNA (SUZ12) retroviral vectors.