Figure 3.

Normal Ig H chain rearrangement but impaired allelic exclusion in PLCγ1^+/−PLCγ2^−/− mice. (A) Normal Ig H chain rearrangement in PLCγ1^+/−PLCγ2^−/− mice. Serial dilutions of genomic DNA extracted from bone marrow cells derived from mice of the indicated genotypes were subjected to two rounds of PCR using primers designed to amplify rearranged V_HJ558DJ_H genes. PCR products were subjected to Southern blot with a fragment spanning JH3 and JH4 as a probe. PCR products from the β-actin gene served as controls for the quantity of the genomic DNA. (B) Impaired Ig H chain allelic exclusion in PLCγ1^+/−PLCγ2^−/− mice. Serial dilutions of genomic DNA extracted from bone marrow cells derived from Ig^HEL BCR transgenic mice of the indicated genotypes were subjected to PCR and Southern blot analysis as described in (A). PCR products from the β-actin gene served as controls for the quantity of the genomic DNA. The figure shown is representative of two independent experiments.