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Wild-type Sly1p and mutant Sly1(L140K)p are localized to the Golgi membranes. Localization of GFP-Sly1p (strain RPY142) and GFP-Sly1(L140K)p (strain RPY220) was analyzed in living cells by fluorescence, or in fixed cells using anti-GFP and anti-Emp47p antibodies. Note the significant colocalization of GFP-Sly1p and GFP-Sly1(L140K)p with the Golgi protein Emp47p.
