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. 2004 Sep 30;23(20):4096–4105. doi: 10.1038/sj.emboj.7600415

Figure 6.

Figure 6

Phosphorylation/activation of CREB (A), MAP kinases (B, C), and CAMKII (D) in Tg mice. (A) Protein extracts were obtained from hippocampii of mice of the indicated genotypes (Tg mAPP, Tg (wt)RAGE, Tg mAPP/RAGE, Tg mAPP/DN-RAGE, nonTg) at 3–4 months of age and subjected to immunoblotting with antibody to total CREB or phospho (p)-CREB. The upper panel displays quantitation of the results with phospho-CREB by image analysis (n=5–8/group) and the lower panel shows representative immunoblots. (B, C) The experiment was performed as in (A), except that antibodies for immunoblotting were anti-total p38 and anti-phospho-p38 IgG (B), and anti-total Erk1/2 and anti-phospho-Erk1/2 IgG (C). The upper panel displays image analysis of results with the phospho antibodies and n=5–8/group (in the case of Erk, image analysis was performed on p44). In panel D, the experiment was performed with antibodies to total and phospho-CAMKII (CAMKII) and the animals were 14–18 months of age (image analysis shows results of phospho-CAMKII from 5–13 animals/group).