Figure 2.
Effects of Gfi1 deletion on the frequencies of HSCs in adult mouse bone marrow. (A) Total bone marrow cells of Gfi1−/− and WT mice were prepared and stained with antibodies against lineage markers (Lin) and the surface markers and receptors Sca-1, c-Kit and Flt3. Lin−Sca-1+c-Kit+ cells (LSK) were analyzed by flow cytometry, gated as previously described to obtain fractions representing HSCs (Akashi et al, 2000; Adolfsson et al, 2001; Christensen and Weissman, 2001) and were reanalyzed for Flt3 expression. (B) The percentages for phenotypically defined HSCs (LSK) and the indicated different HSCs subsets are given with regard to total bone marrow cell numbers. n: number of mice analyzed. P-values were calculated using the Student's t-test.