Table 6.

Gene ontology and enrichment for the effects of temperature in this study. All functional clustering was based on genes with FDR p < .01 and > 1.5‐fold change for each treatment effect and interaction

Comparison	No. genes (No. Annotated)	GO term	Enrich score
Temperature	3,294 (2,490)	1. Electron carrier activity, heme binding, secondary metabolites biosynthesis, transport, and catabolism	5.3****
		2. Endopeptidase inhibitor activity	4.2***
		3. Vitamin binding	2.2**
		4. Carboxylesterase activity	2.1**
		5. Ribosome, histones, cuticular proteins	1.8*
1. 15° to 25° upregulated	254 (194)	1. Endoplasmic reticulum	1.3
2. 15° to 25° downregulated	640 (473)	1. Endopeptidase inhibitor activity	6.4****
		2. Tetrapyrrole binding	2.4**
		3. Hydrolase activity	1.8*
		4. Peptidase activity	1.6*
3. 25° to 35° upregulated	806 (550)	1. Response to temperature	3.8***
		2. Cation binding	1.4*
4. 25° to 35° downregulated	1045 (667)	1. Ribosome	7.5****
		2. Glycolysis/pentose shunt	1.8*
5. 15° to 35° upregulated	962 (710)	1. Response to temperature	3.5***
		2. Cation binding	2.2**
6. 15° to 35° downregulated	1,686 (1331)	1. Endopeptidase inhibitor activity	6.6****
		2. Tetrapyrrole binding	5.3****
		3. Ribosome biogenesis	1.9*
		4. Amino acid biosynthesis	1.4*
7. 15° down to 25° upregulated to 35°	45 (25)	No enriched groups
8. 15° up to 25° downregulated to 35°	52 (37)	No enriched groups
9. 15° up to 25° upregulated to 35°	34 (28)	1. Response to temperature	3.8***
		2. Cation binding	1.4*
10. 15° down to 25° downregulated to 35°	128 (110)	1. Endopeptidase inhibitor activity	4.1***

*p < .05, *^* p < .01, *^** p < .001, *^*** p < .0001.