Table 7.
Gene ontology and enrichment for the effects of region, populations, host cacti on gene expression differences in female Drosophila mojavensis in this study. All functional clustering was based on genes with FDR p < .01 for each treatment effect
| Comparison | No. genes (No. annotated) | GO term | Enrich score |
|---|---|---|---|
| 1. Region | 1432 (954) | 1. Electron carrier activity, heme binding, tetrapyrrole binding, P‐450 gene activity | 6.8**** |
| 2. Endopeptidase inhibitor activity | 2.9** | ||
| 3. tRNA processing | 1.9* | ||
| 4. tRNA aminoacylation | 1.7* | ||
| Mainland > Baja California | 687 (472) | 1. Electron carrier activity, heme binding, tetrapyrrole binding, P‐450 gene activity | 3.2*** |
| 2. tRNA processing | 2.1** | ||
| 3. Cofactor binding | 1.3* | ||
| Baja California > Mainland | 745 (482) | 1. Endopeptidase inhibitor activity | 4.6**** |
| 2. Secondary metabolites biosynthesis, transport, and catabolism, electron carrier activity, heme binding, tetrapyrrole binding | 2.8** | ||
| 3. Exopeptidase activity | 2.0** | ||
| 2. Population | 745 (200) | 1. Peptidase, proteolysis | 7.3**** |
| 2. Secondary metabolites biosynthesis, transport, and catabolism, electron carrier | 2.5** | ||
| 3. tRNA processing | 3.1*** | ||
| 4. Endopeptidase inhibitor activity | 3.0*** | ||
| 5. Nonglucose carbohydrate catabolism | 2.6** | ||
| 3. Baja California | 153 (83) | ||
| Punta Prieta > San Quintin | 72 (39) | 1. Sensory perception | 2.5** |
| 2. Muscle protein | 1.9* | ||
| San Quintin > Punta Prieta | 81 (44) | 1. Calcium ion binding, EGF‐like | 2.1** |
| 2. P‐450 gene activity, oxidoreductase | 1.9* | ||
| 4. Mainland (Arizona vs. Sonora)a | 587 (373) | ||
| Organ Pipe NM > Punta Onah | 204 (133) | 1. Ribosome biogenesis | 2.8** |
| 2. RNA splicing | 2.2** | ||
| 3. Methyltransferase | 2.0** | ||
| 4. Hydro‐lyase activity | 1.5* | ||
| 5. Helicase activity | 1.5* | ||
| Punta Onah > Organ Pipe NM | 383 (240) | 1. EGF‐like region | 4.2*** |
| 2. Extracellular glycosylation, signal peptide | 2.9** | ||
| 3. Extracellular matrix | 2.8** | ||
| 4. Plasma membrane receptor | 2.7** | ||
| 5. Antimicrobial response | 2.7** | ||
| 5. Host cactus | 2457 (1816) | ||
| Agria > Organ pipe | 2094 (1524) | 1. Peptidase, proteolysis | 13.6**** |
| 2. Secondary metabolites biosynthesis, transport, and catabolism, electron carrier activity, heme binding, tetrapyrrole binding | 7.2**** | ||
| 3. ATP, cellular respiration, electron transport | 4.6**** | ||
| 4. Exopeptidase activity | 3.7*** | ||
| 5. Endopeptidase inhibitor activity | 3.7*** | ||
| 6. Cytochrome‐c oxidase activity | 3.6*** | ||
| 7. NADH dehydrogenase activity | 3.2*** | ||
| 8. Extracellular matrix | 2.6** | ||
| 9. Mitochondrial membrane | 2.6** | ||
| 10. TCA cycle | 2.4** | ||
| Organ pipe > Agria | 363 (292) | 1. DNA repair | 9.0**** |
| 2. DNA replication | 8.9**** | ||
| 3. Nucleosome, chromatin assembly | 8.5**** | ||
| 4. ATP binding | 5.0**** | ||
| 5. Mitosis | 2.5** |
*p < .05, **p < .01, ***p < .001, ****p < .0001.
aOrgan Pipe National Monument, Arizona and Punta Onah, Sonora, Mexico.