Figure 2.

Immunofluorescence of the MANF protein in SD rat retina (green for MANF; red for TUJ1 and GFAP). (A) is double labeled for MANF and TUJ1. Images at the top left of the white box show high magnification of the cells indicated by white arrows. Fluorescence staining shows that MANF fluorescence intensity in the cytoplasm is much stronger than in the nucleus. (B) is double labeled for MANF and GFAP. MANF staining is intensely distributed in the cell membrane and cytoplasm in the GCL and with less intensity in the INL. Moderate MANF immunoreactivity is observed in the ONL. MANF is partially co-localized with the glial cell marker GFAP in the GCL (indicated by arrows). The nuclei are labeled with DAPI (blue). Scale bar: 50 μm. ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer.