Table 2.
Plasmids
| Plasmid | Yeast genes | Backbone vector |
|---|---|---|
| pCM-DST1 | CEN TRP1 ptetO7∷DST1 | pCM185 |
| pCM-ΔN | CEN TRP1 ptetO7∷dst1-133,309 | pCM185 |
| pSPT8-84 | 2 μ LEU2 Gal4AD(768–881)∷spt8-334,520 | pACT2 |
| PMED13-111 | 2 μ LEU2 Gal4AD(768–881)∷med13-106,669 | pACT2 |
| pVV70 | 2 μ TRP1 Gal4BD(1–147)∷DST1 | pGBT9 |
| pVV71 | 2 μ TRP1 Gal4BD(1–147)∷dst1-1,132 | pGBT9 |
| pVV72 | 2 μ TRP1 Gal4BD(1–147)∷dst1-1,242 | pGBT9 |
| pVV73 | 2 μ TRP1 Gal4BD(1–147)∷dst1-75,309 | pGBT9 |
| pVV74 | 2 μ TRP1 Gal4BD(1–147)∷dst1-133,309 | pGBT9 |
| pVV75 | 2 μ TRP1 Gal4BD(1–147)∷dst1-243,309 | pGBT9 |
| pVV80 | 2 μ TRP1 pPGK∷DST1 | pGEN |
| pVV81 | 2 μ TRP1 pPGK∷dst1-133,309 | pGEN |
| pVV225 | 2 μ TRP1 pPGK∷SPT8∷13Myc | pVV203 |
| pVV226 | 2 μ TRP1 pPGK∷MED13∷13Myc | pVV203 |
| pVV227 | 2 μ URA3 pPGK∷DST1∷3HA | pVV215 |
| pVV228 | 2 μ TRP1 pPGK∷DST1∷3HA | pVV201 |
| pVV229 | 2 μ TRP1 pPGK∷MED13∷3HA | pVV201 |
| pVV230 | 2 μ TRP1 pPGK∷SPT8∷3HA | pVV201 |
| pVV231 | 2 μ URA3 pPGK∷GCN5∷13Myc | pVV217 |
| pVV232 | 2 μ URA3 pPGK∷SPT7∷13Myc | pVV217 |
| pVV233 | 2 μ URA3 pPGK∷CDK8∷13Myc | pVV217 |
| pVV234 | 2 μ URA3 pPGK∷DST1∷13Myc | pVV217 |
| pYX212-RPB4 | 2 μ URA3 pTPI1∷RPB4 | pYX212 |