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. 2017 Jan 19;8:2. doi: 10.3389/fphar.2017.00002

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Copyright © 2017 Lou, Teng, Zhang, Yang, Ma, Wang, Tian, An, Yang, Zhang, Xu and Dong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TUNEL staining showed the effects of HPβCD/BCP on apoptosis in hippocampal neurons caused by 2VO: sham group (A), 2VO group (B), HPβCD/BCP low dose group (C), HPβCD/BCP middle dose group (D), HPβCD/BCP high dose group (E), and AM630 group (F). The TUNEL positive cells in the 2VO group significantly increased in the CA1 region of hippocampus compared with the sham group. After HPβCD/BCP treatment, the number of TUNEL positive cells was obviously suppressed. And AM630 aggravated apoptosis in the hippocampus compared with the 2VO rats. The bar is 100 μm (^*P < 0.001 vs. sham group, ^##P < 0.001 vs. 2VO group).