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. 2017 Jan 19;8:7. doi: 10.3389/fphys.2017.00007

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Copyright © 2017 Natsiou, Granchi, Mitsiadis and Jimenez-Rojo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Molecular profile of epithelial dentospheres. Immunohistochemistry and immunofluorescence on epithelial dentospheres showing BrdU incorporation in proliferating cells, and expression of specific markers showing the epithelial identity (Krt10, Krt14) and stemness (p63, Sox2) of cells. Absence of Amelogenin indicates that cells within dentospheres are not differentiated into dental specific epithelial cells (ameloblasts). Abbreviations: BrdU, Bromodeoxyuridine; Krt10, Keratin10; Krt14, Keratin14. Scale bars: 30 μm.