Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 19;8:5. doi: 10.3389/fimmu.2017.00005

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Kulkarni, Kurle, Shete, Ghate, Godbole, Madhavi, Kent, Paranjape and Thakar.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Gating strategy for antibody-dependent natural killer (NK) cell activation assay. (A–C) Donor blood (as a source of NK cells) was incubated with the serum/plasma test samples and human immunodeficiency virus (HIV) peptide pools for 5 h and then analyzed by flow cytometry. The lymphocytes were gated using FSC/SSC scatter. The NK cells were identified as CD3−CD56+ cells and assessed for surface CD107a expression and intracellular IFN-γ expression. (D) Representative dot plots for positive control (purified anti-CD16). (E) Unstimulated (DMSO equivalent solution) control. (F,G) Representative displays of ADCC responder (patient-1) and non-responder (patient-2) to HIV-1 Env-C peptide pool.