Table 7.

Primers for PCR amplification of the 12 relatively highly variable regions among the six Lagerstroemia taxa.

No.	Region amplified	Forward primer (5′ → 3′)	Reverse primer (5′ → 3′)	Size (bp)	Annealing temperature (°C)
1	trnK-rps16	TGGGTTCATAGGACTCTATCCA	TTGCAATTGATGTGCGATCTCGA	1202	56
2	trnS-trnG	ACCGAGTTATCAACGGAAACGGA	TAAAGTTTCTGCTCGGAATAAGA	882	53
3	trnG-trnR-atpA	TCTAGAGGGATTATCTAGAAAGCA	AAGAGGTCAACGATTACGTGAGT	975	55
4	trnE-trnT	AGAGGAATGTCCGTTGGG	CGATGACTTACGCCTTACC	1471	53
5	rbcL-accD	TCTCTTAATTGAATTGCAATTCA	AATAGATGAATAGTCATTCGATGA	703	49.5
6	psbL-psbF-psbE	GTGATCCTTCCGAATGGGATAAG	CAGTGAATTTCCATTTACTGATAT	672	51.8
7	trnP-psaJ-rpl33	AGTAGAAGGTTTATATATCTAATA	GATTATTTCGTTGCAATCACAAC	905	48.5
8	rrn16-trnI	TTAGTTGCCACCGGTATGAGAGT	GGTCCTCTTCCCCATTACTTAGA	1845	58
9	ccsA	AGGTATAATCCATGAATATTGAT	TGAATTCATTATAGGACTTATTA	1755	48
10	ndhG-ndhI	ATCGGTTGATAAATGAATTCCAA	CAAGGTTCAATTTGATCTAATCT	790	51
11	rps15-ycf1	TAAGTCTTCGTATCTTATTGGTG	GAGTTTGGATATTCTGATGATTCA	1122	53
12	ycf1	TAACCTCAGCCTTAGCATT	GGACAGAATAGACAAACCCT	2191	50