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(A) Kinetics of F-actin polymerization in the Triton-insoluble, cytoskeletal fraction of wild-type cells. (B) RacB-GTP proteins in wild-type RacB knock-in cells stimulated with cAMP for the indicated time were separated with glutathione–Sepharose beads using the GST-CRIB of PAKa and analyzed by immunoblot assay (see Materials and methods). The values in these and the other experiments are averages based on five separate experiments.
