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Development on non-nutrient agar plates. (A) racB null cells plated at a density of 1.8 × 106 cells/cm2. (B) Cells expressing mutant RacGEF1 proteins plated at a density of 1.8 × 106 cells/cm2. (C) Cells expressing mutant RacGEF1 proteins plated at a density of 2.4 × 105 cells/cm2. (D) Computer-assisted analysis of chemotaxis (DIAS) of strains examined in (A–C).
