Table 2.
DIAS analysis of chemotaxis
| Speed (μm/min) | Directionality | Directional change (deg) | Roundness (%) | |
|---|---|---|---|---|
| Wild type | 9.16±0.45 | 0.89±0.05 | 17.7±3.7 | 55.8±1.5 |
| racB null | 3.88±0.77 | 0.76±0.08 | 23.1±4.6 | 76.1±3.2 |
| gef1 null | 8.23±1.62 | 0.77±0.07 | 24.2±7.6 | 55.3±2.0 |
| GEF1OE | 5.19±0.18 | 0.80±0.07 | 22.7±4.3 | 68.3±5.6 |
| GEF1ΔN | 3.34±0.25 | 0.25±0.07 | 61.5±2.4 | 59.1±4.3 |
| GEF1ΔIQ | 4.13±0.28 | 0.53±0.05 | 47.2±0.7 | 55.8±0.58 |
| GEF1ΔPH | 3.41±0.38 | 0.59±0.12 | 41.7±8.1 | 62.3±1.2 |
| GEF1ΔIQ/ΔPH | 4.61±0.18 | 0.64±0.04 | 38.9±3.8 | 56.5±4.7 |
| Numbers are mean±s.d. Speed indicates speed of cell's centroid movement along the total path. Direction change is a relative measure of the number and frequency of turns the cell makes. Larger numbers indicate more turns and less efficient chemotaxis. Directionality is a measure of the linearity of the pathway. Cells moving in a straight line to the needle have a directionality of 1.00. Roundness is an indication of the polarity of the cells. Larger numbers indicate that the cells are more round and less polarized. | ||||