Figure 2.

MICA is expressed on adherent cells and loss of adhesion downregulates surface expression. (A) Adherent cells (shaded gray histogram) and the cells which had detached from the surface within the cultures (“natural detached,” dashed gray line) were stained for MICA or with an isotype control antibody. (B) Adherent cell lines were cultured over agarose-coated plates for 5 days to force formation of non-adherent spheroids (dashed gray line) or were cultured under non-confluent adherent conditions (shaded gray histogram) and stained for MICA surface expression (isotype control—black line for adherent cells or dashed black line for non-adherent cells). n = 3 independent experiments.