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. 2017 Jan 19;7:687. doi: 10.3389/fimmu.2016.00687

Figure 4.

Figure 4

Extracellular calcium availability modulates MICA surface expression. (A) U2OS cells were cultured either adherently (gray line) or in suspension as spheroids in the presence (dashed gray line; isotype control—dashed black line) or absence of 5 mM EDTA (shaded gray histogram; isotype control—black line) and MICA surface expression analyzed. n = 4. (B) Adherent U2OS, HEK293T, and MCF7 cells were either left untreated (shaded gray histogram; isotype control—black line) or treated with 1 mM (dashed gray line; isotype control—dashed black line) or 5 mM EDTA (dotted gray line; isotype control—dotted black line) and MICA surface expression analyzed. n = 4. (C) Images of adherent U2OS cells treated with 5, 3, 2.5, 2, and 1 mM EDTA for 48 h or left untreated. Bar represents 50 µm. n = 3.