Fig. 1.

Chimeric MtDef4.2 gene expression cassette and its expression in transgenic wheat. a The monocot codon-optimized MtDef4.2 was placed under the control of the constitutively expressing maize Ubi1A promoter/intron/tobacco etch virus (TEV) mRNA leader sequence and 3′ polyadenylation CaMV35S signal terminates transcription. Plant selectable marker gene NPTII is driven by enhanced CaMV35S promoter and 35S polyadenylation is transcription termination sequence. LB left border; P promoter; NPTII neomycin phosphotransferase II; I intron; SP signal peptide; MP mature peptide; RB right border. b Quantitative RT-PCR of MtDef4.2 in the leaf and root tissue of various transgenic wheat lines. Relative expression levels of MtDef4.2 are reported in logarithmic scale and were normalized to expression of wheat ADP glycosylation gene Ta2291. Error bars represent SE of three different biological replicates