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. 2004 Oct 7;23(21):4134–4145. doi: 10.1038/sj.emboj.7600412

Figure 5.

Figure 5

Crosslinking UvrD and MutL by BS3. Coomassie-blue-stained SDS–PAGE gels of UvrD (D) and full-length MutL (L) (A), LN40 (LN) (B), or LC30 (LC) (C) crosslinked by 0.11 mM BS3. The first three lanes are MutL (LN40 or LC30), MutL (LN40 or LC30) mixed with UvrD and UvrD. The following nine lanes are results of crosslinking each set of three protein samples in the presence of AMPPNP and DNA, AMPPNP, or DNA. Molecular-weight standards are indicated. MutL (70 kDa), LN40 (40 kDa) and UvrD (80 kDa), and dimeric LC30 (60 kDa) are labeled. LC30 monomers ran off the gel and are thus absent. The red arrowheads point to the crosslinked bands, whose formation depends on AMPPNP and DNA; the blue arrowheads point to the species crosslinked independent of AMPPNP or DNA.