Fig. 3.

Western blot verification of the expression of chloramphenicol acetyltransferase in the chloroplast of stable chloroplast transformants lines. Transformed cells with pCCATCH by biolistic bombardment (B) and PEG method (P) were analyzed via WB. The presence of CATCH protein was tested in the whole cells (cells) and chloroplast fraction (chlpst) of both varieties. C The stable nuclear transformant pCCATGN with cytosolic localization of the CAT protein was used as a control. Samples were loaded on the SDS–PAGE gel in identical equivalents of chlorophyll a: 0.25 µg of Chla for cells and chloroplasts. The abundance of CAT protein in chloroplasts was comparable with the cellular levels. Neither, the wild type (WT) cells nor the chloroplast fraction of the stable nuclear transformants contained detectable levels of the CAT protein. To confirm that equal amounts of material were loaded on the gel, a chloroplast protein D1 was detected with anti-D1 antibody (lower part)