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. 2004 Oct 7;23(21):4166–4176. doi: 10.1038/sj.emboj.7600427

Figure 5.

Figure 5

The amino-terminal domain of TI-VAMP inhibits FcR and CR-mediated phagocytosis. (A) RAW264.7 macrophages transiently expressing GFP-TI-VAMP, GFP-Longin or GFP-Cdc42N17 were incubated with IgG-SRBCs for 60 min at 37°C. The cells were then fixed and external SRBCs stained with Cy3-anti-rabbit IgG antibodies. The efficiencies of association (left) and phagocytosis (right) were calculated on 50 transfected and 50 control cells. The mean±s.e.m. of nine independent experiments is plotted. Control, GFP-negative cells. (B) RAW264.7 macrophages transiently expressing GFP-TI-VAMP (grey bars) or GFP-Longin (black bars) were allowed to phagocytose zymosan or C3bi-SRBCs for 60 min at 37°C. The samples were processed as in (A). The mean±s.e.m. of three independent experiments is plotted. Control, GFP-negative cells.