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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Dev Neurobiol. 2016 Aug 2;77(4):454–473. doi: 10.1002/dneu.22420

Figure 1.

Figure 1

CSPGs decrease axon branching. (A) Examples of filopodia and branches at different stages of branch formation. F refers to filopodia containing only actin filaments and exhibiting the characteristic linear morphology and actin filament bundles. F+ refers to a filopodium containing a microtubule. NB refers to a nascent branch. Note that the filopodial morphology has changed and the actin filaments are no longer organized in a uniform bundle (compare to examples of F). PB refers to a branchlet which has acquired a polarized distribution of actin filaments, greatest distally. MB refers to mature branches containing a microtubule core and a more prominent distal accumulation of actin filaments resembling a small growth cone. (B) Examples of the cytoskeletal organization of axons on control and CSPG coated substrata. The control example exhibits more axonal filopodia and branches than the axon on CSPGs. (C) Graph showing the distribution of axons exhibiting 0 to ≥4 branches/distal 100 μm on control and CSPG coated substrata. (D) Graph of the number of filopodia along distal axons. (E) Graph of the length of filopodia along distal axons. n = axons in C and D, and filopodia in E.