Figure 3. Depletion of FoxF1 and FoxF2 increased expression of p21^Cip1 and p27^Kip1 in RMS tumors.

RMS tumors were harvested 3 weeks after inoculation of control 76-9 rhabdomyosarcoma cells or 76-9 cells with stable knockdown of FoxF1 or FoxF2 (FoxF1-KD, FoxF2-KD). (A-B) Decreased cellular proliferation was demonstrated by reduced numbers of Ki-67-positive (A) and pH3-positive cells (B). Percentage of Ki-67-positive and PH3-positive cells were counted in five random microscope fields (n=3 mice per group, right panels). A p value < 0.01 is shown with (**). Magnification is 400x (upper panels), 200x (bottom panels). (C) Depletion of FoxF1 or FoxF2 increased expression of p21^Cip1 and p27^Kip1 CDK inhibitors and decreased protein levels of CCND1 and pRb in RMS tumors. Magnification is 200x.