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. Author manuscript; available in PMC: 2017 Feb 10.
Published in final edited form as: Oncogene. 2016 Jul 18;36(6):850–862. doi: 10.1038/onc.2016.254

Figure 5. Depletion of FoxF1 or FoxF2 delays cell cycle progression at the G1/S transition.

Figure 5

(A) Decreased levels of phosphorylated Rb (pRb) protein in FoxF1-KD or FoxF2-KD RMS cells were shown using immunofluorescent staining with antibodies against pRb. Nuclei were counterstained with DAPI. (B) Western blot on FoxF1- or FoxF2-deficient tumor cells showed decreased expression of pRb (Ser780), pRb (Ser795) and pRb (Ser807/811. (C) FoxF1- or FoxF2-depletion caused delay in cell cycle progression at G1/S transition. Control, FoxF1-KD or FoxF2-KD 76-9 RMS cells were serum starved overnight. After serum addition, cells were harvested at 8 hrs, 12 hrs, and 20 hrs. Total DNA content was measured by propidium iodide staining and quantification were done by flow cytometry. (D) Quantification of accumulated FoxF1- or FoxF2-deficient RMS cells in G0/G1 and S phase were performed using flow cytometry analysis. Depletion of FoxF1 or FoxF2 resulted increase accumulation of tumor cells in G0/G1 and decrease number of cells in S phase. Percent of total cells in G1 phase and S phase was presented as mean ± SD. Data represent mean±s.d. of three independent experiments. A p value <0.05 is shown with (*).