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. 2016 Oct 11;92(8):346–357. doi: 10.2183/pjab.92.346

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© 2016 The Japan Academy

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — New setup for odor choice. (A) Schematics of the testing setup. Multiplexed transparent T-maze is placed on the pedestal with infrared LEDs (peak wavelength = 880 nm). Constant airflow (arrows) for odor delivery is created by a blower. The CMOS camera captures the shadows of flies, and captured images are directly saved in a computer. (B) Testing procedures. Flies are loaded into the elevator in the middle of the apparatus. After sliding the elevator the flies are allowed to choose one of the arms. Their choice behavior is filmed by cameras. (C) The testing setup. (D) Image processing. The background image is subtracted to highlight the flies, which can be automatically detected.