Table 1. Myosin heavy chain (MHC) isoforms identified in bovine skeletal muscles by LC-MS/MS.
| Accession no1) | Protein name | MW (Da)/pI2) | Matched peptides | Sequence coverage (%) | Unique peptides |
||||
|---|---|---|---|---|---|---|---|---|---|
| Observed | Mr (expt) | Mr (calc) | Ion score3) | Sequence4) | |||||
| gi|21743235 | MHC 1(2x) | 223900/5.57 | 35 | 18 | 839.1724 | 1676.3299 | 1675.7062 | 103 | QAEEAEEQSNVNLSK |
| 372.9260 | 1115.7557 | 1115.2808 | 43 | VKKQVEQEK | |||||
| 940.3071 | 2817.8990 | 2817.1779 | 46 | MQGTLEDQIISANPLLEAFGNAKTVR | |||||
| 902.1671 | 1802.3194 | 1801.9900 | 53 | TLALLFSGPASGEAEGGPK | |||||
| 675.1037 | 2022.2889 | 2021.1919 | 54 | LAQRLQDAEEHVEAVNAK | |||||
| 821.5016 | 2461.4826 | 2460.7572 | 65 | MQGTLEDQIISANPLLEAFGNAK | |||||
| gi|261245063 | MHC 2(2a) | 224243/5.63 | 37 | 20 | 735.7338 | 2204.1791 | 2203.3206 | 57 | TLEDQVNELKSKEEEQQR |
| 815.8242 | 2444.4503 | 2443.6607 | 46 | SALKTLAFLFSGTPTGDSEASGGTK | |||||
| 755.2849 | 1508.5550 | 1508.6320 | 72 | LQAAEEHVEAVNAK | |||||
| 738.2307 | 2211.6698 | 2210.4856 | 73 | TSVFVAEPKESFVKGTIQSR | |||||
| 808.8453 | 1615.6758 | 1615.7834 | 76 | QLDEKEALVSQLSR | |||||
| gi|13560273 | MHC 7(slow) | 224026/5.58 | 34 | 17 | 737.4436 | 2209.3086 | 2208.4664 | 43 | DVFVPDDKEEFVKATILSR |
| 871.7960 | 1741.5771 | 1740.9532 | 44 | ILNPAAIPEGQFIDSR | |||||
| 372.9751 | 1115.9031 | 1116.3134 | 48 | MFNWMVTR | |||||
| 418.7175 | 1253.1303 | 1252.4630 | 51 | TIHELEKVRK | |||||
| 825.0195 | 1648.0242 | 1646.8570 | 52 | NLTEEMAGLDEIIAK | |||||
| 495.3181 | 1482.9320 | 1482.5517 | 41 | KVQHELDEAEER | |||||
| 776.9608 | 2327.8601 | 2327.5439 | 43 | LKELQARIEELEEELEAER | |||||
| 867.2636 | 2598.7687 | 2597.8355 | 45 | NNLLQAELEELRAVVEQTERSR | |||||
| 618.0512 | 1234.0876 | 1233.3736 | 48 | GQNVQQVVYAK | |||||
| 651.0576 | 1950.1505 | 1949.0416 | 58 | SKAEETQRSVNDLTSQR | |||||
| 1101.1946 | 2200.3744 | 2200.4041 | 77 | GTLEDQIIQANPALEAFGNAK | |||||
| 744.9623 | 1487.9098 | 1487.6591 | 82 | VLSKANSEVAQWR | |||||
| 832.1437 | 1662.2727 | 1661.6796 | 89 | QAEEAEEQANTNLSK | |||||
1)Accession numbers were taken from the NCBI database. 2)Theoretical molecular weight and pI. 3)Ion score >39 indicates identity (p<0.05). 4)Unique peptides specific to each MHC and commonly expressed in bovine LT, PM, SM and ST muscles.