Figure 3.

CDDP suppresses oxygen consumption but does not affect the proton gradient. (A) Intracellular pH of cytosolic pHcyt (○) and mitochondrial pHmit (●) compartments of wild-type cells were determined by monitoring the pH-sensitive protein, pHluorin fluorescence, adjusted for cell density. At t = 0 (arrow), 100 μM CDDP was added. Data are expressed as mean±SD (n=3). (B) Oxygen consumption was determined in wild-type cells buffered at pH 3.0, 5.0 and 7.0 with 0–100 μM CDDP. Data are expressed as percentage of oxygen consumption compared with cells incubated without CDDP (100%) (mean ± SD, n=3)