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. 2016 Nov 29;116(2):218–226. doi: 10.1038/bjc.2016.394

Figure 2.

Figure 2

Effects of PBK/TOPK knockdown by siRNA-PBK/TOPK compared with those of control siRNA in NUGC4 and HGC27 cell lines. (A) shows that the knockdown of PBK/TOPK overexpression in TP53 wild-type NUGC4 cells downregulated expression of pAKT and upregulated that of p21, which resulted mainly in G0–G1 arrest. The proliferation of NUGC4 cell was significantly lower than with controls after the knockdown of endogenous PBK/TOPK expression. However, (B) shows that there was no difference of cell proliferation and FACS results between siRNA-PBK/TOPK and control siRNA cells in TP53 mutant HGC27 cells. The p21 level was not changed, but pAKT level was decreased and PTEN level was increased at 72 h in siRNA-PBK/TOPK-transfected cells. Including the results of Supplementary Figure S2, these results suggested that PBK/TOPK might be related to the cell proliferation in a TP53 mutation-dependent manner.