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. 2017 Jan 5;2017:3692797. doi: 10.1155/2017/3692797

Figure 5.

Figure 5

ROS generation led to apoptosis and autophagy in BZYQD and cisplatin cotreated A549/DDP cells. (a) A549/DDP cells were pretreated with 100, 250, and 500 μg/ml BZYQD for 2 h, and then 40 μg/ml cisplatin was added for another 24 h. After drug cotreatment, cells were labeled with DCFH-DA and the fluorescent intensity of the oxidized product DCF in individual cells was detected by flow cytometry. (b) Statistical analysis of the cell numbers in area P2 in (a). The results are expressed as the mean ± SD of four independent experiments. (ANOVA followed by Bonferroni's test.) (c) A549/DDP cells were pretreated with 2.5 mM NAC 3 h prior to cotreatment with BZYQD and cisplatin. After incubation, cell growth inhibition was determined by the Cell Counting Kit as described previously. The results are expressed as the mean ± SD of four independent experiments. (ANOVA followed by Bonferroni's test.) ROS: reactive oxygen species; BZYQD: Bu-Zhong-Yi-Qi decoction; DCFH-DA: dichlorodihydrofluorescein diacetate; DCF: 20,70-dichlorofluorescein; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; NAC: N-acetylcysteine.