Figure 1.

Intraperitoneal pilocarpine injection leads to stable behavioral seizure activity. (a) After a single dose of intraperitoneal pilocarpine injection (250 mg/kg), behavioral seizure activity was documented every 10 minutes using the modified Racine's stages. Stage 6 animals (indicated by black circles) were not included in the analysis.(stage 0 = no seizures, 1 = freezing, 2 = single twitches, 3 = orofacial seizures, 4 = clonic seizures, 5 = tonic seizures, and 6 = death). (b) 90 minutes after pilocarpine injection 42.3% of the animals were in stages 1–3 and 38.5% were in stage 4 or 5 of the modified Racine's stages. 5 out of 26 animals died during experimental procedure and were not included in further analysis (N = 26 animals; stages 1–3: 11/26 animals, stage 4 or 5: 10/26 animals, death: 5/26 animals).