Figure 4.

Tn7 and Tn3-tagging of the polarity protein Spa2p. The Spa2p protein is indicated as a rectangle. The numbered residues correspond to amino acids; Spa2p is 1466 amino acids in length. The gray shaded region indicates SPA2 coding sequence subjected to transposon mutagenesis. Sequenced Tn7 insertions are shown facing upwards; Tn3 insertions are shown facing downwards. The precise amino acid position of each insertion is indicated below the figure. Bracketed numbers indicate multiple identified transposon insertions at a given site.