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gRNA and Cas9 coexpression vectors. (A) The single vectors (pLdCH and pLdCN) derived from the previous two vectors systems. A2-IGS, A2 intergenic sequence. Please see the Fig. 2 legend for other abbreviation definitions for these vectors. The drawing is not to scale. (B) Miltefosine (MLF) resistance rates of L. donovani cells transfected with the two-vector or single-vector constructs expressing LdMT gRNAa. These are representative data of three independent experiments.
