TABLE 2.
Primers used in the study
| Primer name | Sequence (5′-3′) |
|---|---|
| RSF-DurM-MCSII-up | ATATTAGTTAAGTATAAGAAGGAGATATACATATGGCAGATCTCAATTGGATATCGATGG |
| RSF-DurM-MCSII-dn | GCAGCGGTTTCTTTACCAGACTCGAGGGTACCTTACTGTTCGTGGTGCGACGGCGGGTCGA |
| RSF-DurA-MCSI-up | CAGGATCCGAATTCGAGCTCGGCGCGCCTGACCGCTTCGATTCTTCAGTCCGTCGTGGACG |
| RSF-DurA-MCSI-dn | TGCGGCCGCAAGCTTGTCGACCTGTTACTTGGTGTTGCCGTCACAC |
| PET-DurM-MCSII-up | ATATTAGTTAAGTATAAGAAGGAGATATACATATGGCAGATCTCAATTGGATATCGATGG |
| PET-DurM-MCSII-dn | GCAGCGGTTTCTTTACCAGACTCGAGGGTACCTTACTGTTCGTGGTGCGACGGCGGGTCGA |
| PET-DurA-MCSI-up | CAGGATCCGAATTCGAGCTCGGCGCGCCTGACCGCTTCGATTCTTCAGTCCGTCGTGGACG |
| PET-DurA-MCSI-dn | TGCGGCCGCAAGCTTGTCGACCTGTTACTTGGTGTTGCCGTCACAC |
| ACYC-DurX-MCSII-up | ATTAGTTAAGTATAAGAAGGAGATATACATATGGCCCTGAAGACCTGCGAGGAATTCCTG |
| ACYC-DurX-MCSII-dn | GCGTGGCCGGCCGATATCCAATTGAGATCTGCTCAGCCGTGGTGGCGGAGGGCGTCCATC |
| ACYC-DurN-MCSI-up | TTGTTTAACTTTAATAAGGAGATATACCATGAAAAGCGCCAAGGAACCGACGATCTACCA |
| ACYC-DurN-MCSI-dn | CTGGCTGTGGTGATGATGGTGATGGCTGCTGCCTCAGGACGTCAGCCGGGCGACCAACGT |
| RSF-DurX-MCSII-up | ATTAGTTAAGTATAAGAAGGAGATATACATATGGCCCTGAAGACCTGCGAGGAATTCCTG |
| RSF-DurX-MCSII-dn | GCGTGGCCGGCCGATATCCAATTGAGATCTGCTCAGCCGTGGTGGCGGAGGGCGTCCATC |
| RSF-DurN-MCSI-up | TGTTTAACTTTAATAAGGAGATATACCATGAAAAGCGCCAAGGAACCGACGATCTACCAG |
| RSF-DurN-MCSI-dn | GCGTGGCCGGCCGATATCCAATTGAGATCTGCTCAGCCGTGGTGGCGGAGGGCGTCCATC |
| RSF-DurAT11A-MCSI-dn | TGCGGCCGCAAGCTTGTCGACCTGTTACTTGGTGTTGCCGTCACACACGAAGGCGAACGGGCCGAAGCTGCAGCT |
| RSF-DurAF12A-MCSI-dn | AAGCTTGTCGACCTGCAGGCGCGCCGAGCTTTACTTGGTGTTGCCGTCACACACCGCGGT |
| RSF-DurAV13A-MCSI-dn | AAGCTTGTCGACCTGCAGGCGCGCCGAGCTTTACTTGGTGTTGCCGTCACACGCGAAGGT |
| RSF-DurAC14A-MCSI-dn | AAGCTTGTCGACCTGCAGGCGCGCCGAGCTTTACTTGGTGTTGCCGTCCGCCACGAAGGT |
| RSF-DurAG16A-MCSI-dn | TGCGGCCGCAAGCTTGTCGACCTGTTACTTGGTGTTGGCGTCACACACGAAGGTGAACGGGCCGAAGCTGCAGCT |
| RSF-DurANTK-deletion-MCSI-dn | TGCGGCCGCAAGCTTGTCGACCTGTTAGCCGTCACACACGAAGGTGAACGGGCCGAAGCTGCAGCT |
| His-DurX-MCSI-up | ACCATCATCACCACAGCCAGGATCCGAATTCGATGGCCCTGAAGACCTGCGAGGAATTC |
| His-DurX-MCSI-dn | AAGCTTGTCGACCTGCAGGCGCGCCGAGCTTCAGCCGTGGTGGCGGAGGGCGTCCATCCA |
| PET-DurAF12W, V13S-mcsI-up | GGATCCGAATTCGAGCTCGGCGCGCCTGACCGCTTCGATTCTTCAGTCCGTCGTGGACG |
| PET-DurAF12W, V13S-mcsI-dn | AGCATTATGCGGCCGCAAGCTTGTCGACCTGTTACTTGGTGTTGCCGTCACAGCTCCAGGTGAACGGGCCGAAGCT |
| PET-DurAK2A, Q3N, F12W, V13S-mcsI-up | AGGATCCGAATTCGAGCTCGGCGCGCCTGACCGCTTCGATTCTTCAGTCCGTCGTGGACG |
| PET-DurAK2A, Q3N, F12W, V13S-mcsI-dn | AGCATTATGCGGCCGCAAGCTTGTCGACCTGTTACTTGGTGTTGCCGTCACAGCTCCAGGTGAACGGGCCGAAGCTGCAGCTGTTCGCGCAGGCGAAGGCTTCCGTA |
| PET-DurAF7Y, F10L, K2A, Q3N, F12W, V13S-mcsI-up | CAGGATCCGAATTCGAGCTCGGCGCGCCTGACCGCTTCGATTCTTCAGTCCGTCGTGGACG |
| PET-DurAF7Y, F10L, K2A, Q3N, F12W, V13S-mcsI-dn | AGCATTATGCGGCCGCAAGCTTGTCGACCTGTTACTTGGTGTTGCCGTCACAGCTCCAGGTGAACGGGCCATAGCTGCAGCTGTTCGCGCA |