. 2017 Jan 17;83(3):e02638-16. doi: 10.1128/AEM.02638-16

TABLE 3.

Primers used in this study

Primer no.	Target	Sequence (5′–3′)^a	Restriction enzyme
1^b	fruR	GCGTCTAGAGCTGGGGAGAGGTCATCTGC	XbaI
2^b		TGGCTTGCTTGATGGGAAGCTTAACCTCGCTGATGTTGGAG	HindIII
3^c		ACATCAGCGAGGTTAAGCTTCCCATCAAGCAAGCCATGATC	HindIII
4^c		GCGGCATGCATTACGCTATTCATGCTGATTCTTTCAATC	SphI
5^b	pfkB1	CTCTGTCGACACACCAACTCCAACATCAGC	SalI
6^b		GGGATAGCACCTTCGGAGACTTGATGCCTTTACCACCTGC
7^c		GTCTCCGAAGGTGCTATCCCATTC
8^c		CTCTGTCGACTGGTTGCTGTAGCAACCTGC	SalI
9	ptsI	CTCTCATATGGCTACTGTGGCTGATGTG	NdeI
10		CTCTCATATGTTAGACTGCTGCGTCGATCAC	NdeI
11	SSI3-8	CTCTCCTGCAGGCTCTGGATACTGACGGATG	Sse8387I
12		CTCTCCTGCAGGCTACGACAAGCTATCGGTG	Sse8387I
13	ptsH	CTCTCATATGGCTTCCAAGACTGTAACC	NdeI
14		CTCTCATATGTTTACTCAGCGTCGAGGTC	NdeI
15	SSI9-2	CTCTGTCGACAATCGCTTGACACAATCGGC	SalI
16		CTCTGTCGACTCGTTGGTTGTAATCGACCG	SalI
17	ptsG	CTCTCATATGGCGTCCAAACTGACGAC	NdeI
18		CTCTCATATGTTACTCGTTCTTGCCGTTGAC	NdeI
19	SSI4-3	CTCTGTCGACATTCCTGCGTCTGGTGGTCT	SalI
20		CTCTGTCGACCCGACCAATGATGTAACTGC	SalI
21	ldhA	CTCTCATATGAAAGAAACCGTCGGCAATAAG	NdeI
22		CTCTCATATGTCAGAAGAACTGCTTCTGAATTTC	NdeI
23	SSI4-7	CTCTGTCGACCTGTGGTGACTTTATTGTCTAGG	SalI
24		CTCTGTCGACGCCAGCTTCTGTAAGTAACTC	SalI

The restriction sites used in the cloning procedures are underlined.

Primer used for amplification of the 5′ region of the gene.

Primer used for amplification of the 3′ region of the gene.