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. 2017 Jan 17;83(3):e02660-16. doi: 10.1128/AEM.02660-16

FIG 2.

FIG 2

Schematic illustration of the construction of the vectors used in this study. Primers used in the construction are depicted as blue arrows. apraR, apramycin resistance gene; oriV, pBR origin of replication for E. coli; pArep, remaining nonfunctional part of the origin of replication for Amycolatopsis from plasmid pA387; int3, putative integrase sequence; permE, erythromycin promoter from Saccharopolyspora erythraea; rpsL, gene coding for 30S ribosomal subunit protein S12 from S. erythraea; aacC1, part of a putative acetyltransferase sequence; ΔyagRST, fused flanking region of the yagRST cluster.