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Immunoblot analysis of PSII proteins. (A) SDS-PAGE of total proteins isolated from Synechocystis cells treated with 70.4 mM (4×) NO2− for the indicated periods of time. Each sample corresponds to 10 μg protein. *, protein ladder. (B) Immunoblotting of the samples whose results are depicted in panel A was performed using antibodies specific for D1 and PsbO.
