TABLE 1.
Decay kinetics of flash-induced variable fluorescence in Synechocystisa
| Time (h) | Fast-phase T1 (μs)/A1 (%) | Middle-phase T2 (ms)/A2 (%) | Slow-phase T3 (s)/A3 (%) | A0 (%) |
|---|---|---|---|---|
| Without DCMU | ||||
| 0 | 128 ± 29/36.3 ± 2.8 | 1.3 ± 0.5/38.8 ± 0.3 | 0.06 ± 0.04/17.7 ± 5.8 | 7.2 ± 2.7 |
| 0.5 | 206 ± 11/38.0 ± 1.3 | 3.2 ± 0.1/37.6 ± 0.5 | 0.60 ± 0.11/16.1 ± 0.5 | 8.4 ± 0.3 |
| 1.5 | 242 ± 5/39.4 ± 2.6 | 3.3 ± 0.1/37.8 ± 0.4 | 1.82 ± 0.33/15.7 ± 2.3 | 7.1 ± 0.9 |
| 3 | 226 ± 4/39.1 ± 1.9 | 2.8 ± 0.2/38.8 ± 0.4 | 1.71 ± 0.48/15.8 ± 1.8 | 6.3 ± 0.7 |
| 6 | 198 ± 14/39.2 ± 0.2 | 2.6 ± 0.1/38.2 ± 0.4 | 0.99 ± 0.33/14.7 ± 0.6 | 7.9 ± 0.4 |
| 12 | 136 ± 9/37.8 ± 0.3 | 3.9 ± 0.3/37.3 ± 0.2 | 0.50 ± 0.30/15.8 ± 0.8 | 9.5 ± 1.0 |
| With DCMU | ||||
| 0 | 0.96 ± 0.02/92.0 ± 1.2 | 8.0 ± 1.2 | ||
| 0.5 | 0.96 ± 0.00/91.7 ± 1.0 | 8.3 ± 0.6 | ||
| 1.5 | 1.06 ± 0.02/91.6 ± 1.1 | 8.4 ± 1.1 | ||
| 3 | 1.01 ± 0.03/91.1 ± 0.5 | 8.9 ± 0.5 | ||
| 6 | 1.05 ± 0.11/88.9 ± 1.9 | 11.1 ± 1.9 | ||
| 12 | 1.15 ± 0.24/86.9 ± 2.6 | 13.1 ± 2.6 |
a
Synechocystis cells were treated with 70.4 mM (4×) NO2− for the indicated periods of time, and relaxation of the flash-induced fluorescence yield with or without 20 μM DCMU was measured. The curves were analyzed in terms of three exponential components (fast, middle, and slow phases).