TABLE 1.
Strains used in the study
| Strain | Genotype | Parental strain(s) and/or reference |
|---|---|---|
| W303 | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 | Laboratory of A. Hyman |
| W303 [rho0]a | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 [rho0] | W303 |
| PGAL-SOD1 mutantb | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 PGAL-SOD1::HIS3 | W303 |
| PGAL-SOD1 [rho0] mutanta | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 PGAL-SOD1::HIS3 [rho0] | PGAL-SOD1 mutant |
| PGAL-SOD2 mutantb | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 PGAL-SOD2::HIS3 | W303 |
| PGAL-SOD2 [rho0] mutanta | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 PGAL-SOD2::HIS3 [rho0] | PGAL-SOD2 mutant |
| PGAL-SOD2 Δrtg2 mutantc | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 PGAL-SOD2::HIS3 rtg2::KANMX4 | PGAL-SOD2 mutant; Δrtg2 |
| PGAL-SOD2 Δmks1 mutantb | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 PGAL-SOD2::HIS3 mks1::KANMX4 | Δmks1 mutant |
| PGAL-SOD2 Δyap1 mutantc | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 PGAL-SOD2::HIS3 yap1::KANMX4 | PGAL-SOD2 mutant; Δyap1 |
| Yap1-GFP mutantb | MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3 cdc13-1 YAP1-GFP::HIS3 | W303 |
| Yap1-GFP [rho0] mutanta | MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3 cdc13-1 YAP1-GFP::HIS3 [rho0] | Yap1-GFP mutant |
| Yap1-GFP PGAL-SOD2 mutantc | MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3 cdc13-1 YAP1-GFP::HIS3 PGAL-SOD2::HIS3 | Yap1-GFP mutant; PGAL-SOD2 |
| Yap1-GFP Δrtg2 mutantc | MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3 cdc13-1 YAP1-GFP::HIS3 rtg2::KANMX4 | Yap1-GFP mutant; Δrtg2 |
| Yap1-GFP PGAL-SOD2 Δrtg2 mutantc | MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3 cdc13-1YAP1-GFP::HIS3 PGAL-SOD2::HIS3 rtg2::KANMX4 | Yap1-GFP mutant; Δrtg2 PGAL-SOD2 |
| Yap1-GFP Δmks1 mutantc | MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3 cdc13-1 YAP1-GFP::HIS3 mks1::KANMX4 | Yap1-GFP mutant; Δmks1 (Zyrina et al. [57]) |
| Δyap1 mutantd | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 yap1::KANMX4 | W303 |
| Δrtg2 mutant | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 rtg2::KANMX4 | Zyrina et al. (57) |
| Δyap1 Δrtg2 mutantc | MATa ade2-101 his3-11 trp1-1 ura3-52 can1-100 leu2-3 yap1::KANMX4 rtg2::KANMX4 | Δyap1; Δrtg2 |
| Trx2-GFP mutant | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 TRX2-GFP::HIS3 | Huh et al. (58) |
| Trx2-GFP PGAL-SOD2 mutantc | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 TRX2-GFP::HIS3 PGAL-SOD2::HIS3 | Trx2-GFP mutant; PGAL-SOD2 |
| Trx2-GFP Δrtg2 mutantc | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 TRX2-GFP::HIS3 rtg2::KANMX4 | Trx2-GFP mutant; Δrtg2 |
| Trx2-GFP Δrtg3 mutantc | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 TRX2-GFP::HIS3 rtg3::KANMX4 | Trx2-GFP mutant; Δrtg3 |
| Trx2-GFP Δmks1 mutantc | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 TRX2-GFP::HIS3 mks1::KANMX4 | Trx2-GFP mutant; Δmks1 |
| Gsh1-GFP mutant | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 GSH1-GFP::HIS3 | Huh et al. (58) |
| Gsh1-GFP PGAL-SOD2 mutantc | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 GSH1-GFP::HIS3 PGAL-SOD2::HIS3 | Gsh1-GFP mutant; PGAL-SOD2 |
| Idh1-GFP mutant | MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3 cdc13-1 IDH1-GFP::TRP1 | W303 (courtesy of Iuliia Karavaeva) |
| Idh1-GFP PGAL-SOD2 mutantb | MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3 cdc13-1 IDH1-GFP::TRP1 PGAL-SOD2::HIS3 | Idh1-GFP mutant; PGAL-SOD2 |
| Idh1-GFP Δrtg3 mutantc | MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3 cdc13-1 IDH1-GFP::TRP1 rtg3::KANMX4 | Idh1-GFP mutant; Δrtg3 |
| Idh1-GFP Δmks1 mutantc | MATa ade2-1 trp1-1 can1-100 leu2-3,112 his3-11,15 ura3 cdc13-1 IDH1-GFP::TRP1 mks1::KANMX4 | Idh1-GFP mutant; Δmks1 |
a
Strain produced by ethidium bromide-induced loss of mitochondrial DNA.
b
Strain produced by insertion of the PGAL promoter or GFP tag using a PCR cassette as described by Longtine et al. (56).
c
Strain produced by crossing and tetrad dissection.
d
Strain produced by disruption with a PCR cassette.