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GlcNAc-1-P UTase activity of the wild-type and double-mutant ST0452 proteins
| Protein | Specific activitya (μmol/min/mg protein) |
|---|---|
| ST0452 | 15.08 ± 0.34 |
| G9A/T80A | 0.43 ± 0.01 |
| G9A/Y97A | ND |
| G9A/Y97F | 1.04 ± 0.01 |
| G9A/K147A | 0.21 ± 0.02 |
| T80A/Y97A | 0.48 ± 0.01 |
| T80A/Y97F | 0.60 ± 0.01 |
| T80A/K147A | 1.36 ± 0.02 |
| Y97A/K147A | 1.27 ± 0.04 |
| Y97F/K147A | 2.78 ± 0.08 |
ND, nondetectable. All experiments were repeated three times.
