TABLE 10.
List of primer sets, templates used to construct the wild-type and mutant EcGlmU genes, and names of the product plasmids and proteins
| Forward primer | Reverse primer | Template | Plasmid name | Protein name |
|---|---|---|---|---|
| dEcGlmUF | dEcGlmUR | E. coli genomic DNA | pEcGlmUH | EcGlmU |
| dEcGlmUY103AF | dEcGlmUY103AR | pEcGlmUH | pEcGlmUY103AH | Y103A |
| dEcGlmUY103NF | dEcGlmUY103NR | pEcGlmUH | pEcGlmUY103NH | Y103N |
| dEcGlmUY103VF | dEcGlmUY103VR | pEcGlmUH | pEcGlmUY103VH | Y103V |