TABLE 2.

GlcNAc-1-P UTase and Glc-1-P UTase specific activities of the mutant ST0452 proteins

Protein	Specific activity (μmol/min/mg protein)a
	GlcNAc-1-P UTase	Glc-1-P UTase
ST0452	15.08 ± 0.34	4.08 ± 0.04
Y97A	27.52 ± 0.22	1.74 ± 0.29
Y97C	10.30 ± 0.59	2.18 ± 0.24
Y97D	21.06 ± 0.74	0.66 ± 0.19
Y97E	7.04 ± 0.65	ND
Y97F	18.40 ± 0.69	3.35 ± 0.15
Y97G	18.12 ± 1.32	0.98 ± 0.14
Y97H	43.70 ± 3.63	10.41 ± 0.49
Y97I	18.68 ± 1.36	ND
Y97K	20.37 ± 1.07	1.09 ± 0.07
Y97L	22.03 ± 0.49	9.97 ± 0.54
Y97M	19.51 ± 1.36	11.45 ± 0.81
Y97N	67.11 ± 3.38	16.92 ± 0.58
Y97P	10.31 ± 0.67	ND
Y97Q	34.60 ± 1.64	4.26 ± 0.10
Y97R	ND	ND
Y97S	29.77 ± 1.70	4.51 ± 0.37
Y97T	31.27 ± 0.50	1.03 ± 0.14
Y97V	53.79 ± 0.65	1.22 ± 0.29
Y97W	ND	ND

^aND, nondetectable.