TABLE 3.
Kinetic constants for the GlcNAc-1-P and Glc-1-P UTase activities of the wild-type and Y97 mutant ST0452 proteinsa
| Protein | GlcNAc-1-P Utase activity |
Glc-1-P UTase activity |
||||
|---|---|---|---|---|---|---|
| Km (mM)b | kcat (s−1) | kcat/Km (s−1 mM−1) | Km (mM)c | kcat (s−1) | kcat/Km (s−1 mM−1) | |
| ST0452 | 0.068 ± 0.009 | 9.97 ± 0.36 | 146.6 | 1.23 ± 0.18 | 2.97 ± 0.11 | 2.41 |
| Y97N | 0.147 ± 0.019 | 49.75 ± 1.66 | 338.4 | 2.53 ± 0.36 | 38.15 ± 2.03 | 15.08 |
| Y97M | 0.097 ± 0.020 | 10.90 ± 0.55 | 112.4 | 1.74 ± 0.15 | 14.97 ± 0.46 | 8.60 |
| Y97V | 0.861 ± 0.078 | 48.95 ± 1.21 | 56.9 | 3.28 ± 0.58 | 2.08 ± 0.16 | 0.63 |
a
Assays were carried out in triplicate.
b
The value was determined for GlcNAc-1-P.
c
The value was determined for Glc-1-P.